Phis2-p53

Author: yamt

August undefined, 2024

WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … http://www.biofeng.com/zaiti/jiaomu/p53his.html

新-TF-centered Y1H筛选与转录因子A相互作用的motif实验结题报 …

http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html Webbp53-His2. Catalog No. PVT11251 Packing . p53-His2 Information. Function yeast Hybrid plasmid Resistance Kan Screen / Strain DH5alpha Culture temperature 37degrees … polyethylene repair

Functional characterization of poplar WRKY75 in salt and osmotic ...

http://www.coolaber.com/usr/2160/File/F/F4671.pdf Webb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ … Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His … polyethylene repair strips

Identification and characterization of MYC transcription …

Webb上海北诺生物科技有限公司所提供的酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，质量可靠、规格齐全,上海北诺生物科技有限公司不仅具有专业的技术水平,更有良好的售后服务和优 … Webb酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，. 产品型号：. 产品时间： 2015-11-08. 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯，pQE 载体，原核表达载体，克隆载体，毕赤酵母表达载体，毕赤 ... polyethylene retail carrier bagshttp://www.bnbiotech.com/beinuobio-Products-11104093/ shangri-la official crossword

"WebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... " - Phis2-p53

Phis2-p53

Functional Characterization of Populus PsnSHN2 in Coordinated …

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 …

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http://www.labbase.net/Supply/SupplyItems-4229347.html WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: …

http://www.engineeredprocess.com/images/Honeywell_IFM_P531.P532_IOM.pdf http://www.bnbiotech.com/beinuobio-Products-11104093/

Webb2、酵母双杂交酵母双杂交及系统是一种鉴定和检测蛋白质相互作用的研究方法，因其具有灵敏性高、功能强大、适用范围广等特点，现已被应用于多个研究领域。 ①核蛋白酵母 … Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by …

Webb实验步骤: 1.构建载体目的基因融合连入pGADT7-Rec2 (AD); 顺式作用元件连入pHis2或pHis2.1载体 (顺式元件可以只连接一个拷贝，也可以连接3个串联的并测序确认无误)。. 2.验证两载体分别于对应的空载体共转时，有无激活转录报告基因情况，大多酵母苗株有毒 …

Webb27 apr. 2024 · The glycogen synthase 2 (Gys2) and p53 generates a novel negative feedback circuit in which p53 represses Gys2 expression whereas Gys2 can stabilize … shangri la officeWebb1 dec. 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PagWRKY75 co-transformed yeast cells were used as positive and negative control, respectively. 3.5. Generation of overexpression or knockdown plants for PagWRKY75. shangri-la ocean city mdWebbpHIS2-p53+pGADT7-53m（阳性对照） pHIS2+pGADT7-53m（阴性对照）服务特色：经验足周期短性价比高通量高. 客户提供. 构建酵母文库材料. 细胞样本：细胞数量＞ … shangri la office spaceWebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: … shangri la office tower dubaiWebb3 mars 2024 · The pHIS2-p53/pGADT7-p53 and pHIS2-p53/pGADT7-PsnSHN2 were used as the positive and negative control, respectively. 1, 10 −2 and 10 −4 represent solution dilution ratio of transformed Y187 ... shangri la offeringsWebb15 nov. 2024 · To eliminate the false positive results of the TF-centered Y1H assay, the bait plasmid pGADT7-bZIP44 was verified for self-activation. pGADT7-bZIP44 + pHIS2-p53 and pGAD53m + pHIS2-p53 were respectively transformed into the Y187 yeast cells, which were spread on SD/-Trp-Leu medium and incubated at 30 ℃ for 3–5 d. shangri la online casinoWebbpBIND pACT-MyoD pBIND-Id pG5 luc pCMV-BD pCMV-AD pBD-p53 pBD-NF−κB pAD-SV40T pAD-TRAF pFR-luc pGBKT7 pGADT7 pCL1 pGBKT7-53 pGADT7-T pGBKT7-Lam pACT2 AD Y187 AH109 pSos pMyr pSos MAFB pHis2 pHisSi-1 p53blue pGAG424 pLaczi p53his pGAD53m pBridge Y2H Gold Yeast strain Y187 pIND shangrila ocean isle nc for sale