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WebThe synthetic genes were inserted into a pET22b(+) vector containing a pelB promoter for controlling periplasmic protein expression (Novagen, Madison, WI). For scFv expression, E. coli BL21 (DE3) cells were transformed with recombinant plasmids. WebDescription. Overview. The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET … WebNovagen Business Services · Wisconsin, United States · 34 Employees History Novagen, Inc. was founded in June, 1989 as a company dedicated to providing state-of-the-art products … how to replace battery in toyota key fob 2018