Characterized cre lines
Web(A) An overview of the high-throughput pipeline starting with crosses of mice from every characterized Cre line to a Cre reporter strain (e.g., Ai9, Ai14) to generate double positive mice for ... WebJul 12, 2024 · We also characterized the eight driver lines expressing the DHFR-domain-containing dCre, dgCre, or dgFlpO. ... However, upon crossing Ai109 to 3 Cre lines, Pvalb-IRES-Cre, Nr5a1-Cre and Sst-IRES-Cre, we observed very few GFP-expressing cells in some but not all regions where Cre recombination would normally occur (data not …
Characterized cre lines
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WebCre recombinase activity is expected in white adipose tissue (WAT) and brown adipose tissue (BAT) 012899. STOCK Agrp tm1(cre)Lowl /J. Agrp (mouse) ArGP neurons in the … WebSep 10, 2014 · The utility of recombination in Cre/LoxP animal models is based on the availability of well-characterized Cre driver lines. By controlling the expression of Cre recombinase within a particular type of cell or at a specific development stage, it is possible to control gene deletion in corresponding cells or stages in animal models [ 6 , 7 ].
WebFeb 7, 2024 · Cre, a 38 kDa bacteriophage enzyme, has been shown to catalyze recombination between two 34 bp lox sites. 5 The Cre protein creates a DNA loop and then either excises or inverts the looped segment depending on the orientation of the loxP sites. 6, 7 Initially, the method was developed to function efficiently in yeast to cause … WebJMCRS focuses on maintenance and distribution of the strains, and ensuring their genetic integrity and high health status. For detailed phenotype information characteristics on the majority of strains (including detailed protocols), we …
[email protected]. My work here involves characterizing JAX Cre Repository mice and novel Cre Driver mice in an effort to evaluate these lines for specific as well as previously undefined Cre recombination. Upon annotating these characterized mice at embryonic and adult time points, data is submitted to JAX Mouse Genome Informatics (MGI ... Web1 day ago · Here, we characterized how the RNA transcript from an IBD-associated long non-coding RNA locus (“CARINH-Colitis Associated IRF1 antisense Regulator of Intestinal Homeostasis”) protects against ...
WebThe highest scoring Scrabble word starting with Cre is Crewelworks, which is worth at least 23 points without any bonuses. The next best word starting with Cre is crewing, which is …
WebMethods: Here, we describe a Cre Transgenic (Tg) mini-pig line in which the expression of Cre is directed by a 3-kb 5' fragment of the kidney-specific aquaporin 2 (AQP2); … children\u0027s bean bag chairs on saleWebAug 19, 2024 · Tamoxifen inducible cre mice are a great way to be able to control cre expression with respect to timing. We are going to talk about the do's, the don'ts, and the most frequently asked questions about tamoxifen. Helpful Resources Intraperitoneal Injection of Tamoxifen for Inducible Cre-Driver Lines Characterized Cre Lines Previous … children\u0027s bean bag chairsWebJan 22, 2024 · The four Cre lines generated using TARGATT are PDGF-CreERT2-mCherry ( # 5), MOR23-CreERT2-mCherry ( # 7), GFAP-CreERT2-mCherry ( # 16), and SMHC-CreERT2-mCherry ( # 20). Integration vectors all contain a tissue/cell-specific promoter driving CreERT2-mCherry with an intron and polyA cassette. governor of tawi tawiWebJan 13, 2010 · The key to this tool is the availability of Cre mouse lines with cell- or tissue-type specific expression of Cre recombinase. To date, a large number of Cre-transgenic mouse lines have been generated to target Cre recombinase expression to specific neuronal and glial cell populations in the central nervous system; however, information … children\u0027s bean bags australiaWebHere, we performed comprehensive characterization of this mouse line with quantitative analysis. By crossing this mouse line with Ai14 (RCL-tdTomato), a very sensitive Cre reporter mouse line, we visualized the Cre-expressing cells in various brain regions. governor of tennesseeWebMar 11, 2009 · Here we show embryos resulting from crosses between mice that carry conditional ROSA26-targeted alleles and three different Cre transgenic lines: Sox2-Cre ( 19) for early overall excision in embryo proper ( Figure 2 A), and Nestin-Cre ( 20) or Col2-Cre ( 21) for excision restricted to the nervous system and to bone/cartilage respectively … governor of tayabasWebFeb 1, 2024 · Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26 fstdTom reporter (tdTom) mouse line, we observed that a ... children\u0027s bean bag chairs uk